Seride Robin ve Batgirl’ün yardımıyla şehirde kol gezen kötülüklerle savaşan Gotham Şehri’nin Karanlık Şövalyesi’nin maceraları anlatılıyor. Hikayeler üslup ve konu olarak çizgi romanlara sadık kalmıştır.
Heir to the Wayne family fortune, Bruce Wayne (Kevin Conroy) lives by day as a seemingly lavish playboy millionaire socialite, but by night assumes the role of his crime-fighting alter-ego: the caped crusader known as Batman. Throughout the show, Batman receives help from sidekicks Robin (Loren Lester) and Batgirl (Melissa Gilbert), as well as Police Commissioner Gordon (Bob Hastings), in protecting the streets of Gotham City from a large rogue's gallery of criminals, lunatics, and nemeses.